WebJan 3, 2024 · Rainbow beads are particles that contain a wide range of fluorophores. Optimize cytometer voltages using your samples. To use these beads simply run a voltage series on each PMT typically 300-900 volts and plot the CV of the data versus the voltage range. Beads for better or worse are a sine qua non for the flow. Webcalled Beads. 4. Make a histogram plot for the first fluorescence channel (e.g. BL1-H). 5. Select Beads as the population to display on the histogram. 6. Draw a gate across the entire width of the plot. Preparing Rainbow Beads Dilute one drop of well-mixed rainbow beads in 300 L of flow buffer. Rainbow Initial Set-up Quick Reference 1.
Rainbow Calibration Particles (8 peaks), 3.0 - 3.4 µm - BD
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Rainbow Calibration Particles (8 peaks), 3.0 - 3.4 µm - BD …
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