2024 Phospho 4ebp1 antibody

Phospho 4ebp1 antibody

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August undefined, 2024

Web实验用到的供体是Thr46（铽标记的anti-phospho-4EBP1），受体是绿色荧光标记的4EBP1，是mTOR下游信号通路的蛋白，有活性的mTOR能磷酸化4EBP1，使之与供体结合。如果mTOR活性受到抑制，供体受体距离不能达到荧光能量转移的要求，则在515nm处与485nm处的信号比值增大。 WebMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser65 of mouse 4E-BP1. Background Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E.

4EBP1 Antibodies - Novus Biologicals

WebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. We previously demonstrated that the mammalian target of rapamycin (mTOR) inhibitor rapamycin suppressed orofacial formalin injection-induced nociception; however, the … WebMar 30, 2016 · When 4EBP1, an eIF4E-binding protein, is active (non-p-4EBP1), it binds to eIF4E and hinder formation of the initiation complex and then translation is blocked, favoring apoptosis.... creative food gift setup

Results for "4ebp1" Abcam: antibodies, proteins, kits...

WebProduct Description. Rabbit anti 4EBP1 antibody recognizes an epitope within the C-terminal region (CT) of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1), a rapamycin-sensitive downstream target of TOR (Target of rapamycin), and regulatory factor of eukaryotic mRNA translation. The association between 4EPB1 and eukaryotic ... WebRabbit anti 4EBP1 (pSer65) antibody recognizes eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) also known as phosphorylated heat-and acid-stable protein regulated by insulin 1 (PHAS-I), when phosphorylated at serine 65. 4EBP1 belongs to the eIF4E-binding protein family. 4EBP1, when bound to eIF4E, inhibits cap-dependent ... WebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also detected with immunoblotting. b and c HEK293T and SW480 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h, and the cells were starved for … creative food group tus

Anti-4E-BP1 (phospho Ser65 + Thr70) Antibody (A255095)

LANCE Ultra Europium-anti-phospho-eIF4E-binding protein 1 …

WebThe Phospho-4EBP1 (Thr37/46) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. The first antibody is selected for its specific binding to the phosphorylated motif on the protein, the second for its ability to recognize the protein independent of its phosphorylation state. WebView Rabbit Polyclonal anti-4EBP1 [p Thr46, p Thr37] Antibody (NBP3-13169). Validated Applications: WB. Validated Species: Human, Mouse, Rat + more. Validated by: Biological Strategies, Genetic Strategies. creative food ingredients perry nyWebp-4E-BP1 Antibody (62.Ser 65): sc-293124. Western blot analysis of 4E-BP1 phosphorylation in HeLa (A), K-562 (B) and HEL 92.1.7 (C) whole cell lysates. Description Gene Info Datasheets and Protocols The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5′ end of mRNA. creative food packaging ideas

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Phospho 4ebp1 antibody

WebFigure 3: Anti-4E-BP1 (phospho Ser65 + Thr70) Antibody (AF2308) Western blot analysis of extracts from 293 cells treated with 20% FBS , using Phospho-4EBP1(Ser65/Thr70) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide. WebOur 4EBP1 Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Rat. Use the list below to choose the 4EBP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products.

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Web(orb502277) 4EBP1 (phospho-Ser65/Thr70) antibody (Biotin) - Biorbyt - CiteAb A Biotin-conjugated rabbit polyclonal antibody, raised against Eukaryotic translation initiation factor 4E-binding protein 1 (Mouse), supplied by Biorbyt. Web4EBP1 is a 118 amino acid protein with a predicted molecular weight of 13 kD. Distribution Cytosol and nucleus/ubiquitously expressed Function Regulation of global translation Biology Area Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction Molecular Family Phospho-Proteins Antigen References

WebEach 4EBP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our 4EBP1 Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Rat. WebThe lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -2℃ . When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. 溶质携带物家族-1抗体， ...

WebAll lanes : Anti-eIF4EBP1 (phospho S65) antibody [EPR23889-3] at 1/1000 dilution Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate Lane 2 : HeLa treated with 100nM rapamycin for 24 hours, whole cell whole cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at … WebNov 11, 2008 · To determine whether CDKs could be responsible for the rapamycin-stimulated rephosphorylation of 4EBP1, we treated cells with saturating concentrations of roscovitine (50 μM), a CDK inhibitor. ... Phospho-S6 and actin were blotted for normalization. (D) The experiments were completed in the same way as in C, except WT 4E-BP1, AA 4E …

WebFeb 10, 2024 · Cell Signaling Technology's Phospho-4E-BP1 (Thr70) Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Precipitation, Immunoprecipitation, and Western Blot. Phospho-4E-BP1 (Thr70) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 70.

WebPhospho-4E-BP1 (Ser65) Antibody detects endogenous levels of 4E-BP1 when phosphorylated at serine 65. This antibody has been shown to also recognize 4E-BP1 when phosphorylated at serine 101 (See application reference, Wang et al. 2003). Species … creative food ideasWebThe Simple Western TM antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Size- and Charge-based assays. This database is intended to provide general assay development guidance for identifying and selecting antibodies to test. creative food photography book creative food presentation techniquesWebA Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance research, discovery and detection to improve global health Analytical & Enterprise Solutions An Analytical and Enterprise solutions business, including leading OneSource laboratory services, focused on accelerating scientific outcomes creative foods baltimore ohioWebJun 25, 2010 · Anti-phospho Drosophila S6K, anti-S6K, anti-phospho S6K, anti-Akt, anti-phospho Akt, and anti-phospho 4EBP1 antibodies were from Cell Signaling. Anti-Myc, anti-HA, and anti-FLAG antibodies were from Santa Cruz Biotechnology, Covance, and Sigma, respectively. ... stimulation for 30 min. Phosphorylation and protein levels of dS6K were … creative foods american bbqWebJun 12, 2014 · Total 4EBP1 antibody detects both phosphorylated and non-phosphorylated protein. Samples were treated with lambda-phosphatase or reaction buffer alone and decrease in phosphorylation was noted. X-axis represents iso-electric pH and y-axis represents luminescence units. creative foods burtonWebAbcam - antibodies and reagents supplier, find any antibody Hello. We're improving abcam.com and we'd welcome your feedback. ... 4ebp1 35 results for 4ebp1 ... Anti-eIF4EBP1 (phospho S65) antibody [EPR23889-3] - BSA and Azide free (ab283288) creative foods final exam study guide