Webb11 nov. 2024 · Data shown for (A) through (E) are representative of at least three independent replicates. (F) Amplex Red assay for extracellular H 2 O 2 levels in AsPC-1 cells treated with solvent or IL-4 (50 ng/ml) plus IL-17A (50 ng/ml) for 24 h. H 2 O 2 concentrations were calculated by interpolation from a standard curve, using 0–2 μM H … WebbDescription. The iBlot Transfer Stacks are used to transfer proteins using the iBlot Gel Transfer Device. The iBlot Transfer Stacks are disposable stacks that come in three varieties for different applications: iBlotGel Transfer Stacks have integrated PVDF or nitrocellulose transfer membranes to perform dry blotting of proteins; iBlot DNA …
Addgene: Western Blot
Webb15 nov. 2013 · Learn more at http://www.lifetechnologies.com/iblotThis video shows how to set up and perform western blot protein transfer (dry protein blotting) … Webb24 jan. 2024 · Unseal the iBlot 2 PVDF Mini transfer stack. Set the Top Stack to one side and discard the white separator. Keep the Bottom Stack in the plastic tray. Place the Bottom Stack on the blotting surface. Align the electrical contacts on the blotting surface of the iBlot 2 Gel Transfer Device. brayton richert omaha ne
Supplemental Information DC-SIGN as a Receptor for Phleboviruses
WebbiBlot™ 2 Gel Transfer Device. Ensure the tray is centered so that the electrical contacts are not obstructed. Wet the pre-run gel(s) and place it on the transfer membrane of the … Webb18 nov. 2009 · One of the best features of the iBlot is its footprint. It is not much larger then a midsized horizontal gel chamber. It has its own power supply so the only wire that is needed is from it to the socket. All in all, the iBlot is a great way to optimize protein work. The iBlot itself is inexpensive and the transfer stacks are moderately priced. Webbtransferred with the iBlot 2 device using transfer times of 6–10 minutes at 25 V. 0 5,000 10,000 15,000 20,000 25,000 EGFR (~190 kDa) Transfer time Transfer time Relative band intensity 6 min 8 min 7 min 10 min g g g gg 6 min 7 min 8 min 10 min 20 µg, lane 1 10 µg, lane 2 5 µg, lane 3 2.5 µg, lane 4 1.25 µg, lane 5 iBlot 2 Gel Transfer ... corsodyl mouthwash and amoxicillin