Fccp for seahorse
WebFCCP is an protonophore which is widely used to investigate the role of mitochondria in cellular function. FCCP is a potent uncoupler of mitochondrial oxidative phosphorylation. … WebJun 30, 2024 · The Cell Mito Stress Test is run with six different concentrations of FCCP to determine the optimal FCCP concentration to use in your XF assays. Plate Layout. …
Fccp for seahorse
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Webat -20°C. Oligomycin, FCCP and Antimycin A should be freshly diluted in MAS for each experiment. Stock solutions in DMSO or ethanol (95%) may be stored at -20°C. I.4. Other items needed XF24 Biosensor Cartridge XF24 Tissue Culture Plate Calibration buffer (Seahorse Bioscience) WebApr 7, 2024 · The hypoxia associated with the transforming growth factor-β2 (TGF-β2)-induced epithelial mesenchymal transition (EMT) of human retinal pigment epithelium (HRPE) cells is well recognized as the essential underlying mechanism responsible for the development of proliferative retinal diseases. In vitro, three …
WebAcademia.edu is a platform for academics to share research papers. Web1 Turn on the Seahorse XFe/XF Analyzer, and let it warm up to stabilize. 2 Plate cells at a previously determined density in the Seahorse XF Microplate using the appropriate cell …
WebNov 2, 2024 · Moreover, the FCCP-stimulated respiration was similar between groups, relatively low and insensitive to CPT1 inhibition at all conditions, suggesting that FCCP-induced bioenergetic stress does not trigger increased fatty acid oxidation in E0771 cells. ... Cells were then redistributed in Seahorse XF96 cell culture microplates (at 100,000 cells ... WebApr 14, 2024 · The SEAHORSE cartridge was hydrated with sterile water overnight in a 37 ... port A – oligomycin, 1 µM final concentration, port B – FCCP, 0.3 µM final concentration, port C – rotenone ...
WebDilution volumes for FCCP - Cell Characterization with XFp Ports Used for FCCP Volume of Assay Media (µl) Volume of Stock FCCP (µl) 10X Final FCCP (Port) Concentra-tion (µM) Low Range B, C 487.5 12.5 1.25 D 475 25 2.5 High Range B, C 450 50 5 D 400 100 10 Alternatively, serial dilutions of FCCP may be prepared by add-
WebAt the end of my seahorse assay , each well consists of about 250 ul media (along with the injected drugs). I prepare a stock Hoechst solution in 1:100 dilution in 1X PBS. Once the seahorse run... dove meadows touring caravan parkWebCryptococcus neoformans is a human fungal pathogen that adapts its metabolism to cope with limited oxygen availability, nutrient deprivation and host phagocytes. To gain insight into cryptococcal metabolism, we optimized a protocol for the Seahorse Analyzer, which measures extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) as … dove men body wash clean comfort 1lWebI have started working with the Seahorse XF96 from Agilent starting with the recommended New Cell Line Characterization protocol to determine cell density and FCCP concentration. civil service commission on job orderWebMar 30, 2024 · During set up the assay template there 3 options for Oligomycin injection- Seahorse XF Glycolysis stress test-2, Seahorse mito Stress test-1, Seahorse XF … civil service commission office in batangasWebAug 6, 2024 · Carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone (FCCP) is an uncoupling agent that collapses the proton gradient and disrupts the mitochondrial membrane potential. ... Although the Seahorse ... dove men anti dandruff shampooWebHT29 cells were treated with the indicated compounds ((etoposide, 10 µM; gemcitabine 0.1 µM; paclitaxel 0.01 µM; PD901 1 µM, VX-680 0.2 µM) for 24 hours before analysis of … civil service commission powerWebMar 22, 2024 · 6 answers. Aug 2, 2016. Mito stress Seahorse assay. setting +gene (shcontrol): basal respiration equals to maximal spare capacity (FCCP treatment); … civil service commission of nj