2024 F thp-1 cells

F thp-1 cells

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August undefined, 2024

WebTHP-1 cells were treated with the VCC toxin at 10, 15, 30 and 60 min. LPS (1 µg/mL) was used as a positive control. The VCC starts the transcriptional regulation of subunits of NF-κB, p50. The mRNA expressions were evaluated using qp-PCR. Changes in expression were compared with the control without treatment and normalized with the ... WebAug 10, 2024 · The anti-inflammatory effects and potential mechanisms were investigated in LPS-stimulated THP-1 cells, using salvianolic acid B (SalB) as the positive control. The enzyme-linked immunosorbent assays (ELISA) were used to determine the secretory protein levels of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α ...

M1 and M2 macrophages derived from THP-1 cells ... - BMC Cancer

WebPublished protocols using the promonocytic cell line THP-1 have tended to result in cells that closely resemble classically-activated macrophages, differentiated in IFNγ and LPS. However, no protocol, to date, has fully recapitulated polarization of THP-1 to the M(IL-4) or M(IL-10) macrophage phenotypes seen when human monocyte-derived ... WebJan 13, 2024 · FIGURE 1. Figure 1. The morphologies of THP-1 monocytes and their derived macrophage-like cells at resting stage (M0) and immature dendritic-like cells (iDC) can be distinguished from each other. THP-1 … breezeline basic cable

THP-1 Cell Counter, THP-1 Cells Molecular Devices

WebAug 8, 2015 · Here, we investigated the effects of M2 in comparison to M1 macrophages on cancer cell sensitivity to etoposide. Methods: We set up a model of macrophage polarization, starting from THP-1 monocytes differentiated into macrophages using PMA (Phorbol 12-myristate 13-acetate). Once differentiated (M0 macrophages), they were … WebAug 4, 2003 · F-actin in THP-1 cells was detected with FITC-conjugated phalloidin, which was then observed under a fluorescence microscope, quantified with use of a … WebTHP-1. TIB-202 ™. THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder research, immunology research, and toxicology … breezeline basic cable channels

DUSP1 mediates BCG induced apoptosis and inflammatory response in THP-1 ...

THP-1 - TIB-202 ATCC

WebFeb 21, 2024 · THP-1 cells (2 × 10 5) were seeded and Staphylococcus aureus or Escherichia coli particles labeled with pHrodo red and pHrodo green were added. After 1 h of incubation at 37°C, cells were washed, stained with DAPI and analyzed using flow cytometry. THP-1 cells showed a concentration-dependent uptake of S. aureus(A & B) … WebJan 11, 2024 · f HL60 cells were treated with RSL3 (1 μM) for different hours and then cocultured with THP-1 cells. The ratio of lipid peroxidation (stained with Liperfluo) was measured by flow cytometry. breezeline billing address to send paymentWebMar 3, 2024 · Briefly, for migration assay, 200 μL of cell suspensions without FBS (for THP-1 cells, 1 × 10 5 cells pretreated with 100 ng/mL PMA (Sigma-Aldrich, P8139) for 24 hours and rested in RPMI 1640 with 10% FBS for another 24 hours; for KFs and NFs, 3 × 10 4 cells pretreated with 500 nM AS1517499 (or DMSO control) were added to the upper 24 … breezeline atlantic broadband uniontown pa

"WebJan 12, 2024 · Mastering the Art of Growing THP-1 cells. Published January 12, 2024. Posted in: Cells and Model Organisms. Tissue culture can sometimes seem like a black art. Too careful—your cells go down. Not … " - F thp-1 cells

F thp-1 cells

Mastering the Art of Growing THP-1 cells - Bitesize Bio

WebJan 18, 2024 · 鲍曼不动杆菌外膜囊泡诱发人单核-巨噬细胞THP-1的炎性反应. 1.扬州市第三人民医院，江苏扬州 225000； 2. 江苏大学医学院，江苏镇江 212013. 鲍曼不动杆菌 (Acinetobacterbaumannii,A.baumannii)是目前临床常见病原菌，近年来各类抗生素的长期大量使用，鲍曼不动杆菌 ... WebAbstract. A new macrophage cell line with activated character and unique morphology was isolated by selecting adherent cells from the human monocytic cell line THP-1. The …

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WebMay 3, 2024 · e THP-1 cells were treated with an ATPR concentration gradient (10 −5 –10 −9 M) for 72 h. Then, the protein expression of cyclin A2, CDK4, cyclin D3 and p-Rb was assessed by western blot. f THP-1 cells were treated with ATPR (10 −6 M) at different time points (0–72 h). Then, the protein expression of cyclin A2, CDK4, cyclin D3 and p ... WebNov 16, 2024 · THP-1 cells (ATCC TIB-202) were cultured in RPMI1640 [final concentrations: 4.5 g/L glucose, 2 mM L-GlutaMAX TM, 10 mM HEPES and 1 mM sodium pyruvate] containing 10% (v/v) FBS and 0.05 mM 2-mercaptoethanol. THP-1 monocytes were terminally differentiated using 100 nM phorbol 12-myristate 13-acetate (PMA) for 24 h.

WebAbstract. THP-1 is a human leukemia monocytic cell line, which has been extensively used to study monocyte/macrophage functions, mechanisms, signaling pathways, and … WebOct 7, 2024 · THP-1 cells (ATCC® TIB202™) were cultured using RPMI-1640 (Life Technologies) supplemented with 2 mM L-glutamine, 10% FCS and Penstrep (100 U/ml) at 37°C, 5% CO 2.Cells were grown to a density of 5 × 10 5 –1 × 10 6 cells/ml and used for experiments between passage 5 and 12. For THP-1 compound treatment and …

WebApr 14, 2024 · Cell culture. MOLM13, MV411 and THP-1 cells were obtained from the Sanger Institute cancer cell collection and were grown in 1640-RPMI (Gibco, 21875034) … Web3.1.2.1 Labeling effector cells and target cells. 1. Count THP-1 cells. If cell number allows, duplicate or triplicate wells are set up for each condition. 2. Count the target cells. Take …

WebWe incubated unprimed THP-1 cells either in high K + containing media or pre-treated these cells with broad-spectrum chloride channel inhibitor NPPB (50 μM), or NBC19: a …

WebInvivoGen’s THP-1 cells also respond to NOD1, RIG-I, MDA-5 and STING/cGAS agonists. ... (PMA, catalog #tlrl-pma) induces differentiation of THP1 cells into adherent … could you please advise 意味WebAt the Cell Counter: THP-1 Cells. Derived from the blood of an acute monocytic leukemia patient over 30 years ago, the human monocytic cell line THP-1 is widely used to study … breezeline business hoursWebApr 20, 2024 · F THP-1 cells were transfected with two pairs of MCL1 siRNAs for 24 h, then treated with 8 μM cytarabine for 24 h, and the levels of γ-H2A.X and p-Chk1 were … breezeline business customer serviceWebRemove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. could you please advise us on the below emailWebThe cell fate of THP-1-differentiated macrophages shows heterogeneity of cell growth and apoptosis. 18 Through an MTT cell proliferation assay, which can measure the reduction of tetrazolium into an insoluble formazan product in viable cells, 19 PMA-stimulated THP-1 cells significantly (p < 0.05) exhibited a decrease in the cell growth rate . could you please advise us on how to proceedWebFeb 14, 2024 · (F) THP-1 cells were transfected with siRNA-NC (negative control) or siRNA-DUSP1 for 24 h, and then infected with BCG and cultured for 6 h. The expression levels of p-p38, p-ERK, p-JNK and p-NF ... could you please alsoWebApr 8, 2024 · f, TMEM173 −/− THP-1 cells stably expressing STING-WT or STING-E 336 G/E 337 G (EE/GG) were infected with VACV for the indicated times and analysed by western blot. could you please an appointment