Web疏水层析也称疏水作用层析(hydrophobic interaction chromatography HIC)。蛋白质表面一般有疏水与亲水基团,疏水层析是利用蛋白质表面某一部分具有疏水性,与带有疏水性的载体在高盐浓度时结合。在洗脱时,将盐… WebMar 26, 2024 · butyl: [noun] any of four isomeric alkyl radicals C4H9− derived from butane.
HiTrap Butyl FF Cytiva
WebProduct Support. HiTrap Butyl HP are prepacked Butyl Sepharose High Performance columns for high-resolution hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elution conditions. Provide convenient and fast small-scale protein purifications. WebRituximAb - Proteomix HIC Butyl NP5 Column Length Impact - 4610 vs. 4603. Column: Proteomix ® HIC Butyl NP5, 5 µm, 4.6 x 35 mm, 4.6x 100 mm (PN: 431NP5-4603, PN: 431NP5-4610) Mobile phase: A: 100 mM sodium phosphate buffer, 2 M ammonium sulfate, pH 7.0, B: 100 mM sodium phosphate buffer, pH 7.0; christa mcauliffe astronaut training
Intact and Oxidized Monoclonal Antibody Separation on …
Webresins) with ether, phenyl, butyl, or hexyl functional groups. Both TOYOPEARL and TSK-GEL HIC resins use 1000 Å pore size base resin. Summary of HIC resin range TSK-GEL bulk polymeric media in 20 and 30µm particle size: Ether-5PW Phenyl-5PW TOYOPEARL bulk polymeric media in 35, 65 and 100µm particle size: Ether-650 Phenyl-650 Butyl-650 WebHIC is used in the biopharmaceutical industry for the analysis of antibody drug conjugates (ADCs) or to determine the aggregate content of monoclonal antibodies. TSKgel ® HIC … WebHydrophobic interaction chromatography (HIC) HIC is commonly used for aggregate removal and has been shown to be highly effective [37, 38]. HIC is typically run in FT mode for this purpose. Aggregates are generally more hydrophobic than monomer, and an appropriate condition can be chosen under which monomer flows through whereas … geometry chart chemistry