2024 Atga2ox8基因过表达载体构建及遗传转化植株表型分析

Atga2ox8基因过表达载体构建及遗传转化植株表型分析

Author: chzv

August undefined, 2024

WebApr 29, 2010 · Gibberellin 2-oxidase (GA 2-oxidase) plays very important roles in plant growth and development. In this study, the AtGA2ox8 gene, derived from Arabidopsis (Arabidopsis thaliana), was transformed and over-expressed in rapeseed (Brassica napus L.) to assess the role of AtGA2ox8 in biomass accumulation and lignification in plants. … WebApr 28, 2024 · Abstract. Gibberellin (GA) hormones regulate the development of plants and their responses to environmental signals. The final part of GA biosynthesis is catalyzed by multifunctional 2-oxoglutarate-dependent dioxygenases, which are encoded by multigene families. According to their enzymatic properties and physiological functions, GA …

OsGA2ox5, a Gibberellin Metabolism Enzyme, Is Involved in …

Both genes encode enzymes that have functional similarities to AtGA2ox7 and AtGA2ox8, which are class III GA 2-oxidases that 2β-hydroxylate C20-GAs. Previously unknown for GA 2-oxidases, AtGA2ox9 performs 2α-hydroxylation of C19-GAs and harbors putative desaturating activity of C20-GAs. WebFeb 15, 2011 · Six C20 GA2ox genes, except for AtGA2ox7 and AtGA2ox8, were detected. Recent research demonstrated that improvements in plant architecture, such as semi-dwarfism, increased root systems and higher tiller numbers could be induced by overexpression of wild-type or modified C20 GA2ox in rice (Lo et al., 2008). C20 GA2ox … books 2 year old

ATGA2OX8 - PrimePCR Assay and Template Life Science Bio …

WebOct 30, 2024 · Key message Using a fixed RIL population derived from a widely used foxtail millet backbone breeding line and an elite cultivar, we constructed a high-density bin map and identified six novel multi-environment effect QTLs and seven candidate genes for dwarf phenotype. Abstract Plant height is an important trait that determines tradeoffs between … WebNov 12, 2024 · The GA2ox gene, which belongs to a small multigene family, encodes GA 2-oxidase, the major catabolic enzyme in plants that converts the active GA to an inactive … WebUse Bio-Rad's PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed. books4cars

The Multifunctional Dioxygenases of Gibberellin Synthesis - OUP …

Comprehensive expression analysis of Arabidopsis GA2

WebWe identified two genes in Arabidopsis (AtGA2ox7 and AtGA2ox8), using an activation-tagging mutant screen, that encode 2beta-hydroxylases. GA levels in both activation … books4all.netWebWe found that CsGA2ox8, a gene whose product catalyzes the deactivation of bioactive GAs, was signiﬁcantly upregulated in treatment X1 compared to treatment X2. This expression difference was conﬁrmed by quantitative reverse … books2door free delivery code

"WebAug 3, 2010 · 2012-03-26 基因的克隆,转导,表达,在对目的基因pcr时,为什么一些人设... 8 2011-08-24 你好，请问我是要提取一段目的基因，后续要表达蛋白，在引物设计... 1 2012 … " - Atga2ox8基因过表达载体构建及遗传转化植株表型分析

Atga2ox8基因过表达载体构建及遗传转化植株表型分析

ATGA2OX8 - PrimePCR Assay and Template Life Science Bio …

WebOct 25, 2024 · Plant height is an important trait in crops such as rice, maize, wheat, and rapeseed (Brassica napus L.) (Ashikari et al. 1999; Ji-hua et al. 2007; Würschum et al. 2015; Li et al. 2016).The stems of tall rice, wheat, and rapeseed plants are not strong enough to bear the grain weight of high-yielding varieties, so the identification and introduction of … WebExpression levels of the transgenes in LMP1::AtGA2ox2 and LMX5::AtGA2ox8 plants. (a) AtGA2ox2 expression in different organs of LMP1::AtGA2ox2 plants. (b) AtGA2ox8 …

Did you know?

WebOverexpression of both AtGA2ox7 and AtGA2ox8 caused dwarfing ( Figures 1C and 1D), indicating that the substrates for these enzymes are required for normal elongation … WebAtGA2ox7 and AtGA2ox8 are more similar to each other than to other proteins encoded in the Arabidopsis genome, indicating that they may constitute a separate class of GA …

WebAtGA2ox7 and AtGA2ox8 are more similar to each other than to other proteins encoded in the Arabidopsis genome, indicating that they may constitute a separate class of GA … WebNov 26, 2010 · The AtGA2ox8 (Genbank accession number: AL021960; Tair Web site accession number: At4g21200) gene from Arabidopsis thaliana cDNA was amplified using a reverse-transcription polymerase chain reaction (RT–PCR) method and specific 5′ (5′-TCC CCCGGG ATGGATCCACCATTCAACGAAATA T -3′) and 3′ (5′-CCG CTCGAG …

WebApr 11, 2024 · Jo-Anne Theresa Glassock. July 4, 1946 - March 20, 2024 Dearly beloved Jo-Anne Theresa Glassock passed away on March 20, at the age of 76, in Laguna … WebJul 13, 2024 · Both genes encode enzymes that have functional similarities to AtGA2ox7 and AtGA2ox8, which are class III GA 2-oxidases that 2β-hydroxylate C 20 -GAs. Previously unknown for GA 2-oxidases, AtGA2ox9 performs 2α-hydroxylation of C 19 -GAs and harbors putative desaturating activity of C 20 -GAs.

WebJan 1, 2024 · The homolog of CsGA2ox8 in Arabidopsis is AtGA2ox8, whose product functions in hydroxylating the C 20-GA precursors GA 12 and GA 53 15. In this study, consistent with the results from overexpression of AtGA2ox8 15, GA 12 and GA 53 levels dramatically decreased in OE8.1, suggesting that CsGA2ox8 has a function similar to …

WebAug 1, 2024 · GA2-oxidases (GA2oxs) are a class of 2-oxoglutarate-dependent dioxygenases that regulate the deactivation of bioactive GAs. In this study, we investigated the phylogeny and domain structures of the seven GA2ox genes present in the Arabidopsis thaliana genome. books4africaWebJan 1, 2024 · We found that CsGA2ox8, a gene whose product catalyzes the deactivation of bioactive GAs, was significantly upregulated in treatment X1 compared to treatment X2. This expression difference was... godzilla\\u0027s city crossword clueWebJan 27, 2014 · The overexpression of AtGA2ox7 and AtGA2ox8 produce a dwarf phenotype with reduced GA levels, while ectopic expression of AtGA2ox7 and AtGA2ox8 in … godzilla\u0027s parents react to himWebJan 27, 2014 · The overexpression of AtGA2ox7 and AtGA2ox8 produce a dwarf phenotype with reduced GA levels, while ectopic expression of AtGA2ox7 and AtGA2ox8 in transgenic tobacco ( Nicotiana tabacum) leads to a dwarf phenotype [22]. A similar phenotype was also observed in rice overexpressing OsGA2ox6 [25]. books43 freeWebNov 1, 2013 · Morphological alterations by overexpression of the GA2ox gene have already been reported for various plant species: transgenic A. thaliana and N. tabacum plants containing AtGA2ox7 or AtGA2ox8 from A. thaliana exhibited dwarf phenotypes (Schomburg et al., 2003); transgenic Solanum melanocerasum and S. nigrum plants … godzilla\\u0027s parents react to himWebMar 20, 2014 · Gibberellin (GA), a plant hormone, is involved in many aspects of plant growth and development both in vegetative and reproductive phases. GA2-oxidase plays a key role in the GA catabolic pathway to reduce bioactive GAs. We produced transgenic Arabidopsis plants expressing GA2-oxidase 4 (AtGA2ox4) under the control of a … books 4 africa canterburyWebDec 14, 2010 · Gibberellins (GAs) are endogenous hormones that play an important role in regulating plant stature by increasing cell division and promoting seed germination. The GA2-oxidase gene from Arabidopsis thaliana (AtGA2ox8) was introduced into Brassica napus L. by Agrobacterium-mediated floral-dip transformation with the aim of decreasing … books 3 for 2